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KMID : 0364219800230040263
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Korean Journal of Zoology
1980 Volume.23 No. 4 p.263 ~ p.272
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Studies on the Change of Isozyme Patterns of Lactate and Malate Dehydrogenases During Embryonic Development of Some Amphibians
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Park Young-Chul
Ha Doo-Bong
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Abstract
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Polyacrylamide gel electrophoresis was used to investigate the patterns of LDH and MDH isozymes in the embryo and adult of amphibia; Rana nigromaculata, Rana plancyi chosenica and Hynobius leechii. Rana nigromaculata is considered to be heterozygous for the gene specifying the "B" subunit of LDH, and Hynobius leechii to be heterozygous for the gene specifying the "A" subunit of LDH. The LDH isozyme paatern of embryos of the above three species is characterized by a gradual increase in the activity of LDH-5 (muscular form)during development. Two or three molecular forms of MDH is present steadily from early embryos and in adult. Of the MDH isozymes, the more cathodic one (MDH-m) appears weakly in early developing stages, but increases slowly in the activity as the embryo develops.the embryo develops.
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